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生物物理与神经生物学前沿学术报告 Minhua Luo

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生物物理与神经生物学前沿学术报告

? ? ? ?Frontiers in Biophysics and Neurobiology?

报告题目:?Neurotropic Herpesviruses and the Derivate Anterograde neuronal circuit Tracers

报告人: ?Min Hua Luo, Ph.D

? ? ? ? ? ? ? ? ? ? Professor of Wuhan Institute of Virology Chinese Academy of Sciences

时间: ? 2018年1月8日(周一),下午:14:00-15:00

地点:  西区生物楼531会议室

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中国科学技术大学生命科学学院

合肥微尺度物质科学国家实验室集成影像中心

报告简介:

Herpes simplex virus type 1 strain 129 (H129) represented ?a?potential?anterograde neuronal circuit tracing tool, which complements ?the existing retrograde tracers. However, the current H129 derived ?tracers are multisynaptic, neither bright enough to label the details of ?neurons nor capable of determining direct projection targets ?as?a?monosynaptic tracer.?Here we?introduce an improved multisynaptic ?anterograde tracer H129-G4 with strong labeling intensity, and a novel ?mono-synaptic anterograde tracer H129-ΔTK-tdT. H129-G4 is capable of ?transmitting through multiple synapses, labeling the neurons by green ?florescent protein, and visualizing the morphological details of the ?labeled neurons. H129-ΔTK-tdT neither replicates nor spreads in neurons ?alone, but transmits to and labels the postsynaptic neurons with ?tdTomato in the presence of complementary expressed TK from a helper ?virus. H129-ΔTK-tdT is also capable to map the direct projectome of the ?specific neuron type in the given brain regions in Cre transgenic mice. ?The H129-ΔTK-tdT anterograde monosynaptic tracing system offers a useful ?tool for mapping the direct output in neuronal circuitry. H129-G4 is an ?anterograde multisynaptic tracer with a labeling signal strong enough ?to display the details of neuron morphology.

How to improve the tracers, how viral particles traffic in axon and how to display the tracing efficiency are undergoing study.

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生物物理与神经生物学前沿学术报告 Minhua Luo

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